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验证数据展示

  • IHC staining of human rectal cancer using 84292-4-RR

    IHC staining of human rectal cancer using 84292-4-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84292-4-RR (ADAM17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ADAM17 antibody included
  • FC experiment of HCT 116 using 84292-4-RR

    FC experiment of HCT 116 using 84292-4-RR

    1x10^6 HCT 116 cells were intracellularly stained with 0.25 ug ADAM17 Recombinant antibody (84292-4-RR, Clone:241305A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 84292-4-RR

    WB analysis using 84292-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84292-4-RR (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84292-4-RR

    Affinity and Kinetic Characterization of 84292-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84292-4-RR against Human ADAM17 were performed. The affinity constant is 0.385 nM.

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ADAM17 Recombinant monoclonal antibody

ADAM17 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 84292-4-RR

产品说明书

CloneNo. 241305A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, FC (Intra), ELISA

别名

241305A2, ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, HCT 116 cells, HUVEC cells, SW480 cells, HeLa cells, RAW 264.7 cells
Positive IHC detected inhuman rectal cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHCT 116 cells
Planning an IHC experiment? We recommend our IHCeasy ADAM17 Ready-To-Use IHC Kit. ADAM17 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84292-4-RR targets ADAM17 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32418

Product name: Recombinant human ADAM17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 693-824 aa of BC136783

Sequence: CVDKKLDKQYESLSLFHPSNVEMLSSMDSASVRIIKPFPAPQTPGRLQPAPVIPSAPAAPKLDHQRMDTIQEDPSTDSHMDEDGFEKDPFPNSSTAAKSFEDLTDHPVTRSEKAASFKLQRQNRVDSKETEC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ADAM metallopeptidase domain 17
别名 241305A2, ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86
计算分子量 824 aa, 93 kDa
观测分子量100-130 kDa
GenBank蛋白编号BC136783
基因名称 ADAM17
Gene ID (NCBI) 6868
RRIDAB_3671836
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP78536
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

实验方案

Product Specific Protocols
WB protocol for ADAM17 antibody 84292-4-RRDownload protocol
IHC protocol for ADAM17 antibody 84292-4-RRDownload protocol
FC protocol for ADAM17 antibody 84292-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols