Beta Actin Monoclonal antibody
Beta Actin Monoclonal Antibody for FC, IHC, WB, ELISA
Cat No : 60008-1-Ig
|Positive WB detected in||multi-cells/tissue, HeLa cells, MCF-7 cells, HEK-293 cells, A549 cells, rice whole plant tissue, arabidopsis whole plant tissue|
|Positive IHC detected in||human colon tissue, human brain tissue, human kidney tissue, human lung tissue, human spleen tissue, human ovary tissue, human testis tissue, human placenta tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HepG2 cells|
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60008-1-Ig targets Beta Actin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, plant, Zebrafish samples.
|Tested Applications||FC, IHC, WB, ELISA|
|Cited Applications||CoIP, IF, IHC, IP, WB|
|Tested Reactivity||human, mouse, rat, plant, Zebrafish|
|Cited Reactivity||human, rat, cat, goat, tick, chicken, yeast, Arabidopsis, Terminalia bellirica, Rare minnow|
|Immunogen||Beta Actin fusion protein Ag0297 种属同源性预测|
|Host / Isotype||Mouse / IgM|
|Full Name||actin, beta|
|Synonyms||ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin|
|Calculated molecular weight||375 aa, 42 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC002409|
|Gene ID (NCBI)||60|
|Purification Method||Caprylic acid/ammonium sulfate precipitation|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
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