Beta Actin Monoclonal antibody
Beta Actin Monoclonal Antibody for FC, IHC, WB, ELISA
Cat No : 60008-1-Ig
|Positive WB detected in||multi-cells/tissue, HeLa cells, MCF-7 cells, HEK-293 cells, A549 cells, rice whole plant tissue, arabidopsis whole plant tissue|
|Positive IHC detected in||human colon tissue, human brain tissue, human kidney tissue, human lung tissue, human spleen tissue, human ovary tissue, human testis tissue, human placenta tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
60008-1-Ig targets Beta Actin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, plant, Zebrafish samples.
|Tested Applications||FC, IHC, WB, ELISA|
|Cited Applications||CoIP, IF, IHC, IP, WB|
|Tested Reactivity||human, mouse, rat, plant, Zebrafish|
|Cited Reactivity||Arabidopsis, Artemia sinica, Caenorhabditis elegans, canine, cat, cattle, chicken, Dendrobium nobile, duck, goat|
|Immunogen||Beta Actin fusion protein Ag0297|
|Host / Isotype||Mouse / IgM|
|Full Name||actin, beta|
|Calculated molecular weight||375 aa, 42 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC002409|
|Gene ID (NCBI)||60|
|Purification Method||Caprylic acid/ammonium sulfate precipitation|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
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