Beta Actin Monoclonal antibody, PBS Only

Beta Actin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 60008-1-PBS

产品说明书

CloneNo. 7D2C10

宿主/亚型

Mouse / IgM

种属反应性

human, mouse, rat, zebrafish, plant

应用

WB, IHC, IF/ICC, IF-P, ELISA

Actin, ACTB, bate actin, β actin, Beta-actin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


Planning an IHC experiment? We recommend our IHCeasy ACTB Ready-To-Use IHC Kit. ACTB primary antibody included.

产品信息

60008-1-PBS targets Beta Actin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, zebrafish, plant
免疫原 Beta Actin fusion protein Ag0297 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 actin, beta
别名 Actin, ACTB, bate actin, β actin, Beta-actin
计算分子量 375 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC002409
基因名称 Beta Actin
Gene ID (NCBI) 60
RRIDAB_2289225
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP60709
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

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