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验证数据展示

  • WB analysis of rat liver using 86982-1-RR (same clone as 86982-1-PBS)

    WB analysis of rat liver using 86982-1-RR (same clone as 86982-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 86982-1-RR (ACSL5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86982-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse liver using 86982-1-RR (same clone as 86982-1-PBS)

    WB analysis of mouse liver using 86982-1-RR (same clone as 86982-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 86982-1-RR (ACSL5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86982-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 86982-1-RR (same clone as 86982-1-PBS)

    IHC staining of human liver using 86982-1-RR (same clone as 86982-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86982-1-RR (ACSL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86982-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL5 antibody included
  • IHC staining of human liver using 86982-1-RR (same clone as 86982-1-PBS)

    IHC staining of human liver using 86982-1-RR (same clone as 86982-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86982-1-RR (ACSL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86982-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL5 antibody included
  • Affinity and Kinetic Characterization of 86982-1-RR

    Affinity and Kinetic Characterization of 86982-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86982-1-RR against Human ACSL5 were performed. The affinity constant is below 1 pM.

ACSL5 Recombinant monoclonal antibody, PBS Only

ACSL5 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86982-1-PBS

产品说明书

CloneNo. 252098G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

ACSL 5, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.3, FACL5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ACSL5 Ready-To-Use IHC Kit. ACSL5 primary antibody included.

产品信息

86982-1-PBS targets ACSL5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8258

Product name: Recombinant human ACSL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 388-739 aa of BC007985

Sequence: DIRLLADDMKTLKPTLFPAVPRLLNRIYDKVQNEAKTPLKKFLLKLAVSSKFKELQKGIIRHDSFWDKLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAAMGCQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNNEGEVCIKGTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLPNGTLKIIDRKKNIFKLAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSFEELCQNQVVREAILEDLQKIGKESGLKTFEQVKAIFLHPEPFSIENGLLTPTLKAKRGELSKYFRTQIDSLYEHIQD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-CoA synthetase long-chain family member 5
别名 ACSL 5, Arachidonate--CoA ligase, EC:6.2.1.15, EC:6.2.1.3, FACL5
计算分子量 76 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC007985
基因名称 ACSL5
Gene ID (NCBI) 51703
RRIDAB_3745254
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULC5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACSL5 (acyl-CoA synthetase long-chain family member 5) is a gene encoding an enzyme that activates long-chain fatty acids into acyl-CoA esters, thereby directing them toward lipid synthesis or β-oxidation. It is predominantly expressed in the small intestine, uterus, and spleen, with minimal presence in normal brain tissue. ACSL5 has gained clinical attention for its marked upregulation in malignant gliomas, where it promotes fatty-acid-driven tumor growth, and its mutations are linked to congenital diarrhea (DIAR13). Additionally, its expression levels are being explored as prognostic markers in colorectal cancer and as modulators of apoptotic signaling.

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