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验证数据展示

  • IF Staining of human prostate cancer using CL488-60176

    IF Staining of human prostate cancer using CL488-60176

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 ACPP antibody (CL488-60176, Clone: 1G2C9 ) at dilution of 1:200.

  • IF Staining of HeLa using CL488-60176

    IF Staining of HeLa using CL488-60176

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ACPP antibody (CL488-60176, Clone: 1G2C9 ) at dilution of 1:200, CL594-phalloidin (red).

CoraLite® Plus 488-conjugated ACPP Monoclonal antibody

ACPP Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-60176

产品说明书

CloneNo. 1G2C9

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat

应用

IF/ICC, IF-P

别名

PAP, ACP 3, acid phosphatase, prostate, Acid phosphatase 3, 5'-nucleotidase

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inhuman prostate cancer tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PAP/ACPP Ready-To-Use IHC Kit. PAP/ACPP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60176-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60176 targets ACPP in IF/ICC, IF-P applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag8578

Product name: Recombinant human ACPP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 27-373 aa of BC007460

Sequence: DRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELAGPVIPQDWSTECM

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 acid phosphatase, prostate
别名 PAP, ACP 3, acid phosphatase, prostate, Acid phosphatase 3, 5'-nucleotidase
计算分子量 418 aa, 48 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC007460
基因名称 ACPP
Gene ID (NCBI) 55
RRIDAB_2919233
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15309
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ACPP antibody CL488-60176Download protocol
Standard Protocols
Click here to view our Standard Protocols
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