验证数据展示
经过测试的应用
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-60176 targets ACPP in IHC applications and shows reactivity with human, rat samples.
| 经测试应用 | IHC Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag8578 Product name: Recombinant human ACPP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 27-373 aa of BC007460 Sequence: DRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELAGPVIPQDWSTECM 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | acid phosphatase, prostate |
| 别名 | PAP, 1G2C9, 5'-NT, 5'-nucleotidase, Acid phosphatase 3 |
| 计算分子量 | 418 aa, 48 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC007460 |
| 基因名称 | ACPP |
| Gene ID (NCBI) | 55 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P15309 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin ACPP antibody Biotin-60176 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
