验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67509 targets Aconitase 2 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | Aconitase 2 fusion protein Ag17784 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | aconitase 2, mitochondrial |
| 别名 | ACO2, Citrate hydro-lyase, Aconitase2, Aconitase, ACO 2 |
| 计算分子量 | 85 kDa |
| 观测分子量 | 85 kDa |
| GenBank蛋白编号 | BC014092 |
| 基因名称 | ACO2 |
| Gene ID (NCBI) | 50 |
| RRID | AB_3084773 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q99798 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 Aconitase 2 antibody CL594-67509 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |