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验证数据展示

  • WB analysis using 67509-1-Ig

    WB analysis using 67509-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human liver cancer using 67509-1-Ig

    IHC staining of human liver cancer using 67509-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACO2 antibody included
  • IHC staining of human liver cancer using 67509-1-Ig

    IHC staining of human liver cancer using 67509-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACO2 antibody included
  • FC experiment of HeLa using 67509-1-Ig

    FC experiment of HeLa using 67509-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACO2 (67509-1-Ig, Clone:1F1G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 67509-1-Ig

    WB analysis using 67509-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67509-1-Ig

    WB analysis of HEK-293 using 67509-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Aconitase 2 Monoclonal antibody

Aconitase 2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67509-1-Ig

产品说明书

CloneNo. 1F1G4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, FC (Intra), ELISA

别名

ACO2, Citrate hydro-lyase, Aconitase2, Aconitase, ACO 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-12 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HEK-293 cells, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue, Neuro-2a cells, rabbit brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ACO2 Ready-To-Use IHC Kit. ACO2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67509-1-Ig targets Aconitase 2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Aconitase 2 fusion protein Ag17784 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aconitase 2, mitochondrial
别名 ACO2, Citrate hydro-lyase, Aconitase2, Aconitase, ACO 2
计算分子量 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC014092
基因名称 ACO2
Gene ID (NCBI) 50
RRIDAB_2882730
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99798
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).

实验方案

Product Specific Protocols
WB protocol for Aconitase 2 antibody 67509-1-IgDownload protocol
IHC protocol for Aconitase 2 antibody 67509-1-IgDownload protocol
FC protocol for Aconitase 2 antibody 67509-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols