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验证数据展示

  • WB analysis using 22670-1-AP (same clone as 22670-1-PBS)

    WB analysis using 22670-1-AP (same clone as 22670-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)

    IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)

    IHC staining of human stomach cancer using 22670-1-AP (same clone as 22670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 22670-1-AP (same clone as 22670-1-PBS)

    IHC staining of human stomach using 22670-1-AP (same clone as 22670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 22670-1-AP (A4GNT antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22670-1-PBS in a different storage buffer formulation.

A4GNT Polyclonal antibody, PBS Only

A4GNT Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22670-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

EC:2.4.1.-, Alpha4GnT, Alpha-1,4-N-acetylglucosaminyltransferase

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

22670-1-PBS targets A4GNT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18432

Product name: Recombinant human A4GNT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 76-340 aa of BC119639

Sequence: KIYPEWPVVFFMKGLTDSTPMPSNSTYPAFSFLSAIDNVFLFPLDMKRLLEDTPLFSWYNQINASAERNWLHISSDASRLAIIWKYGGIYMDTDVISIRPIPEENFLAAQASRYSSNGIFGFLPHHPFLWECMENFVEHYNSDIWGNQGPELMTRMLRVWCKLEDFQEVSDLRCLNISFLHPQRFYPISYREWRRYYEVWDTEPSFNVSYALHLWNHMNQEGRAVIRGSNTLVENLYRKHCPRTYRDLIKGPEGSVTGELGPGNK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alpha-1,4-N-acetylglucosaminyltransferase
别名 EC:2.4.1.-, Alpha4GnT, Alpha-1,4-N-acetylglucosaminyltransferase
计算分子量 340 aa, 39 kDa
观测分子量 34-37 kDa
GenBank蛋白编号BC119639
基因名称 A4GNT
Gene ID (NCBI) 51146
RRIDAB_11183768
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNA3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

A4GNT (alpha-1,4-N-acetylglucosaminyltransferase) catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans, forming a unique glycan structure, GlcNAcalpha1-->4Galbeta-->R. The gene is expressed with a bias in certain tissues, particularly in the stomach (RPKM 9.5) and duodenum (RPKM 0.9). Alpha4GnT has been implicated in O-glycan biosynthesis and is thought to have a role in preventing gastric cancer by inhibiting Helicobacter pylori infection and suppressing tumor-promoting inflammation.

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