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验证数据展示

  • FC experiment of Transfected HEK-293T using 98555-1-RR

    FC experiment of Transfected HEK-293T using 98555-1-RR

    1x10^6 HEK-293T cells or CCL7/MCP-3 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL7/MCP-3 Rabbit RecAb (98555-1-RR, Clone: 250662G6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • FC experiment of Transfected HEK-293T using 98555-1-RR

    FC experiment of Transfected HEK-293T using 98555-1-RR

    1x10^6 HEK-293T cells or CCL7/MCP-3 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL7/MCP-3 Rabbit RecAb (98555-1-RR, Clone:250662G6) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • Affinity and Kinetic Characterization of 98555-1-RR

    Affinity and Kinetic Characterization of 98555-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98555-1-RR against Mouse CCL7/MCP-3 were performed. The affinity constant is 0.524 nM.

Anti-Mouse CCL7/MCP-3 Rabbit Recombinant Antibody

CCL7/MCP-3 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98555-1-RR

产品说明书

CloneNo. 250662G6

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

C-C motif chemokine 7, Ccl7, Fic, Intercrine/chemokine MARC, Mcp3

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
PE
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98555-1-RR targets CCL7/MCP-3 in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原

CatNo: Eg4626

Product name: Recombinant Mouse CCL7/MCP-3 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 24-97 aa of NM_013654.3

Sequence: QPDGPNASTCCYVKKQKIPKRNLKSYRRITSSRCPWEAVIFKTKKGMEVCAEAHQKWVEEAIAYLDMKTPTPKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 7
别名 C-C motif chemokine 7, Ccl7, Fic, Intercrine/chemokine MARC, Mcp3
计算分子量11kDa
GenBank蛋白编号NM_013654.3
基因名称 Ccl7
Gene ID (NCBI) 20306
RRIDAB_3746175
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ03366
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

The chemokine CCL7 (MCP3) is a chemotactic factor and potent attractant of monocytes firstly characterized from the culture supernatants of MG-63 osteosarcoma cells. CCL7 is known to promote the recruitment of many innate immune cell types including monocytes and neutrophils to sites of bacterial and viral infection and eosinophils and basophils to sites of allergic inflammation. CCL7 is expressed at low levels in endothelial cells, fibroblasts and mononuclear cells and upregulated by various stimuli including viruses, type I or type II interferons (IFNs). CCL7 (MCP-3) mediates effects on a host of innate and adaptive immune cell types through binding to numerous receptors including CCR1, CCR2, CCR3, CCR5, and CCR10.

实验方案

Product Specific Protocols
FC protocol for CCL7/MCP-3 antibody 98555-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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