验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Transfected HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.1 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-98555 targets CCL7/MCP-3 in FC (Intra) applications and shows reactivity with mouse samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg4626 Product name: Recombinant Mouse CCL7/MCP-3 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 24-97 aa of NM_013654.3 Sequence: QPDGPNASTCCYVKKQKIPKRNLKSYRRITSSRCPWEAVIFKTKKGMEVCAEAHQKWVEEAIAYLDMKTPTPKP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 7 |
| 别名 | C-C motif chemokine 7, Ccl7, Fic, Intercrine/chemokine MARC, Mcp3 |
| 计算分子量 | 11kDa |
| GenBank蛋白编号 | NM_013654.3 |
| 基因名称 | Ccl7 |
| Gene ID (NCBI) | 20306 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q03366 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
The chemokine CCL7 (MCP3) is a chemotactic factor and potent attractant of monocytes firstly characterized from the culture supernatants of MG-63 osteosarcoma cells. CCL7 is known to promote the recruitment of many innate immune cell types including monocytes and neutrophils to sites of bacterial and viral infection and eosinophils and basophils to sites of allergic inflammation. CCL7 is expressed at low levels in endothelial cells, fibroblasts and mononuclear cells and upregulated by various stimuli including viruses, type I or type II interferons (IFNs). CCL7 (MCP-3) mediates effects on a host of innate and adaptive immune cell types through binding to numerous receptors including CCR1, CCR2, CCR3, CCR5, and CCR10.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CCL7/MCP-3 antibody PE-98555 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
