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验证数据展示

  • FC experiment of human PBMCs using 98506-5-RR

    FC experiment of human PBMCs using 98506-5-RR

    1x10^6 untreated or anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TGF beta 1 Rabbit RecAb (98506-5-RR, Clone: 250119E2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then fixed and stained with Anti-Human Foxp3. CD4+ cells were pre-gated for analysis.

  • FC experiment of human PBMCs using 98506-5-RR

    FC experiment of human PBMCs using 98506-5-RR

    1x10^6 anti-CD3/CD28 treated human PBMCs were surface stained with 0.25 ug Anti-Human TGFB1 Rabbit RecAb (98506-5-RR, Clone: 250119E2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human TGFB1 Rabbit RecAb (98506-5-RR, Clone: 250119E2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). CD4+ cells were pre-gated for analysis. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98506-5-RR

    Affinity and Kinetic Characterization of 98506-5-RR

    Biolayer interferometry (BLl) kinetic assays of 98506-5-RR against Human TGF beta 1 were performed. The affinity constant is below 1 pM.

Anti-Human TGF beta 1 Rabbit Recombinant Antibody

TGF beta 1 Uni-rAb® Recombinant Antibody for FC
Cat No. 98506-5-RR

产品说明书

CloneNo. 250119E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, Latency-associated peptide, TGF beta

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inAnti-CD3/CD28 treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98506-5-RR targets TGF beta 1 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg0879

Product name: Recombinant Human TGF beta 1 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 30-390 aa of

Sequence: LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transforming growth factor, beta 1
别名 TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, Latency-associated peptide, TGF beta
计算分子量44 kDa
基因名称 TGFB1
Gene ID (NCBI) 7040
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01137
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.

实验方案

Product Specific Protocols
FC protocol for TGF beta 1 antibody 98506-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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