验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
98466-3-RR targets GITR/TNFRSF18 in IF, FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | mouse |
| 文献引用反应性 | mouse |
| 免疫原 | GITR/TNFRSF18 fusion protein Eg3695 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor receptor superfamily, member 18 |
| 别名 | CD357, GITR, Tnfrsf18, AITR, Glucocorticoid-induced TNFR-related protein |
| 计算分子量 | 25kDa |
| GenBank蛋白编号 | NM_009400.2 |
| 基因名称 | CD357 |
| Gene ID (NCBI) | 21936 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O35714-1 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for GITR/TNFRSF18 antibody 98466-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |