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验证数据展示

  • WB analysis using 87741-1-RR (same clone as 87741-1-PBS)

    WB analysis using 87741-1-RR (same clone as 87741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87741-1-RR (MLKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87741-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03218-1

    Sandwich ELISA sample test of MP03218-1

    The mean MLKL concentration was determined to be 6.73 ng/mL in HUVEC cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA sample test of MP03218-1

    Sandwich ELISA sample test of MP03218-1

    Plasma of six individual healthy human donors was measured. The human MLKL concentration of detected samples was determined to be 2.02 ng/mL with a range of 0.22 - 6.91 ng/mL.

  • Sandwich ELISA standard curve of MP03218-1

    Sandwich ELISA standard curve of MP03218-1

    Sandwich ELISA standard curve of MP03218-1, Human MLKL Recombinant Matched Antibody Pair - PBS only. 87741-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15188. 87741-2-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL

MLKL Recombinant monoclonal antibody, PBS Only (Capture)

MLKL Uni-rAb® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 87741-1-PBS

产品说明书

CloneNo. 253011B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Sandwich ELISA, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MLKL Ready-To-Use IHC Kit. MLKL primary antibody included.

产品信息

87741-1-PBS targets MLKL as part of a matched antibody pair:

MP03218-1: 87741-1-PBS capture and 87741-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15188

Product name: Recombinant human MLKL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-263 aa of BC028141

Sequence: MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQSLESSSGKSPLEISRFKVKNVKTGSASGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mixed lineage kinase domain-like
别名
计算分子量 471 aa, 54 kDa
观测分子量55 kDa
GenBank蛋白编号BC028141
基因名称 MLKL
Gene ID (NCBI) 197259
RRIDAB_3745725
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8NB16
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MLKL is a pseudokinase effector of necroptosis that becomes activated through RIPK3-mediated phosphorylation, leading to oligomerization and plasma membrane disruption. By executing necroptotic cell death and promoting release of inflammatory mediators, MLKL plays a central role in innate immunity, inflammatory pathology, and host defense. Dysregulation of MLKL-dependent necroptosis has been linked to inflammatory disorders, infection, and cancer-related immune responses.

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