验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87741-1-RR targets MLKL in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag15188 Product name: Recombinant human MLKL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-263 aa of BC028141 Sequence: MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQSLESSSGKSPLEISRFKVKNVKTGSASGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | mixed lineage kinase domain-like |
| 别名 | hMLKL, Mixed lineage kinase domain-like protein |
| 计算分子量 | 471 aa, 54 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC028141 |
| 基因名称 | MLKL |
| Gene ID (NCBI) | 197259 |
| RRID | AB_3745725 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8NB16 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MLKL is a pseudokinase effector of necroptosis that becomes activated through RIPK3-mediated phosphorylation, leading to oligomerization and plasma membrane disruption. By executing necroptotic cell death and promoting release of inflammatory mediators, MLKL plays a central role in innate immunity, inflammatory pathology, and host defense. Dysregulation of MLKL-dependent necroptosis has been linked to inflammatory disorders, infection, and cancer-related immune responses.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MLKL antibody 87741-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
