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验证数据展示

  • WB analysis of 4T1 using 87468-1-RR (same clone as 87468-1-PBS)

    WB analysis of 4T1 using 87468-1-RR (same clone as 87468-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 87468-1-RR (ST6GAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87468-1-PBS in a different storage buffer formulation.

  • IF Staining of 4T1 using 87468-1-RR (same clone as 87468-1-PBS)

    IF Staining of 4T1 using 87468-1-RR (same clone as 87468-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 4T1 cells using ST6GAL1 antibody (87468-1-RR, Clone: 251963F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87468-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 87468-1-RR (same clone as 87468-1-PBS)

    IHC staining of mouse liver using 87468-1-RR (same clone as 87468-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 87468-1-RR (ST6GAL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87468-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03142-1

    Sandwich ELISA sample test of MP03142-1

    Serum of six mice was measured. The mouse ST6GAL1 concentration of detected samples was determined to be 1.60 ng/mL with a range of 0.34 - 4.13 ng/mL.

  • Sandwich ELISA standard curve of MP03142-1

    Sandwich ELISA standard curve of MP03142-1

    Sandwich ELISA standard curve of MP03142-1, Mouse ST6GAL1 Recombinant Matched Antibody Pair - PBS only. 87468-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg5251. 87468-2-PBS was HRP conjugated as the detection antibody. Range: 62.5-2000 pg/mL

  • Affinity of 87468-1-RR

    Affinity of 87468-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87468-1-RR against Mouse ST6GAL1 were performed. The affinity constant is 0.19 nM.

Mouse ST6GAL1 Recombinant monoclonal antibody, PBS Only (Capture)

ST6GAL1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 87468-1-PBS

产品说明书

CloneNo. 251963F1

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

Alpha 2,6-ST 1, Beta-galactoside alpha-2,6-sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, EC:2.4.3.1, Sialyltransferase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87468-1-PBS targets ST6GAL1 as part of a matched antibody pair:

MP03142-1: 87468-1-PBS capture and 87468-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg5251

Product name: Recombinant mouse ST6GAL1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 27-403 aa of NM_001252505.1

Sequence: KKGSDYEALTLQAKVFQMPKSQEKVAVGPAPQAVFSNSKQDPKEGVQILSYPRVTAKVKPQPSLQVWDKDSTYSKLNPRLLKIWRNYLNMNKYKVSYKGPGPGVKFSVEALRCHLRDHVNVSMIEATDFPFNTTEWEGYLPKENFRTKAGPWHKCAVVSSAGSLKNSQLGREIDNHDAVLRFNGAPTDNFQQDVGTKTTIRLVNSQLVTTEKRFLKDSLYTEGILILWDPSVYHADIPQWYQKPDYNFFETYKSYRRLHPSQPFYILKPQMPWELWDIIQEISPDLIQPNPPSSGMLGIIIMMTLCDQVDIYEFLPSKRKTDVCYYHQKFFDSACTMGAYHPLLFEKNMVKHLNEGTDEDIYLFGKATLSGFRNNRC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 beta galactoside alpha 2,6 sialyltransferase 1
别名 Alpha 2,6-ST 1, Beta-galactoside alpha-2,6-sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, EC:2.4.3.1, Sialyltransferase 1
计算分子量47 kDa
观测分子量39-42 kDa and 49-50 kDa
GenBank蛋白编号NM_001252505.1
基因名称 St6gal1
Gene ID (NCBI) 20440
RRIDAB_3745566
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ64685
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ST6GAL1 is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta, and skeletal muscle (PMID: 15049997, PMID: 23358684). Two molecular forms of ST6GAL1 are generally observed, a larger 49-50 kDa and a smaller 39-42 kDa form, representing its full-length and the smaller soluble catalytic domain, respectively (PMID: 35676533, PMID: 35661210).

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