验证数据展示
经过测试的应用
| Positive WB detected in | 4T1 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | 4T1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87468-1-RR targets ST6GAL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Eg5251 Product name: Recombinant mouse ST6GAL1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 27-403 aa of NM_001252505.1 Sequence: KKGSDYEALTLQAKVFQMPKSQEKVAVGPAPQAVFSNSKQDPKEGVQILSYPRVTAKVKPQPSLQVWDKDSTYSKLNPRLLKIWRNYLNMNKYKVSYKGPGPGVKFSVEALRCHLRDHVNVSMIEATDFPFNTTEWEGYLPKENFRTKAGPWHKCAVVSSAGSLKNSQLGREIDNHDAVLRFNGAPTDNFQQDVGTKTTIRLVNSQLVTTEKRFLKDSLYTEGILILWDPSVYHADIPQWYQKPDYNFFETYKSYRRLHPSQPFYILKPQMPWELWDIIQEISPDLIQPNPPSSGMLGIIIMMTLCDQVDIYEFLPSKRKTDVCYYHQKFFDSACTMGAYHPLLFEKNMVKHLNEGTDEDIYLFGKATLSGFRNNRC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | beta galactoside alpha 2,6 sialyltransferase 1 |
| 别名 | Alpha 2,6-ST 1, Beta-galactoside alpha-2,6-sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, EC:2.4.3.1, Sialyltransferase 1 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 39-42 kDa and 49-50 kDa |
| GenBank蛋白编号 | NM_001252505.1 |
| 基因名称 | St6gal1 |
| Gene ID (NCBI) | 20440 |
| RRID | AB_3745566 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q64685 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ST6GAL1 is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta, and skeletal muscle (PMID: 15049997, PMID: 23358684). Two molecular forms of ST6GAL1 are generally observed, a larger 49-50 kDa and a smaller 39-42 kDa form, representing its full-length and the smaller soluble catalytic domain, respectively (PMID: 35676533, PMID: 35661210).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ST6GAL1 antibody 87468-1-RR | Download protocol |
| IHC protocol for ST6GAL1 antibody 87468-1-RR | Download protocol |
| WB protocol for ST6GAL1 antibody 87468-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
