验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, NCI-H1299 cells, HepG2 cells, HCT 116 cells, SW480 cells, mouse testis tissue, rat testis tissue |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:100-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87130-1-RR targets MTFR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag10857 Product name: Recombinant human MTFR1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-333 aa of BC036116 Sequence: MLGWIKRLIRMVFQQVGVSMQSVLWSRKPYGSSRSIVRKIGTNLSLIQCPRVQFQINSHATEWSPSHPGEDAVASFADVGWVAKEEGECSARLRTEVRSRPPLQDDLLFFEKAPSRQISLPDLSQEEPQLKTPARANEEALQKICALENELAALRAQIAKIVTQQEQQNLTAGDLDSTTFGTIPPHPPPPPPPLPPPALGLHQSTSAVDLIKERREKRANAGKTLVKNNPKKPEMPNMLEILKEMNSVKLRSVKRSEQDVKPKPVDATDPAALIAEALKKKFAYRYRSDSQDEVEKGIPKSESEATSERVLFGPHMLKPTGKMKALIENVSDS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | mitochondrial fission regulator 1 |
| 别名 | Chondrocyte protein with a poly-proline region, CHPPR, FAM54A2, KIAA0009 |
| 计算分子量 | 333 aa, 37 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC036116 |
| 基因名称 | MTFR1 |
| Gene ID (NCBI) | 9650 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15390 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTFR1, also known as CHPPR, is a mitochondria protein. It may play a role in mitochondrial aerobic respiration. It may also regulate mitochondrial organization and fission.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MTFR1 antibody 87130-1-RR | Download protocol |
| WB protocol for MTFR1 antibody 87130-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
