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验证数据展示

  • WB analysis of Raji using 87097-1-RR (same clone as 87097-1-PBS)

    WB analysis of Raji using 87097-1-RR (same clone as 87097-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 87097-1-RR (CR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87097-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02864-2

    Sandwich ELISA sample test of MP02864-2

    The mean CD21 concentration was determined to be 152.00 ng/mL in Daudi cell extract based on a 1.8 mg/mL extract load.

  • Sandwich ELISA sample test of MP02864-2

    Sandwich ELISA sample test of MP02864-2

    Plasma of six individual healthy human donors was measured. The human CD21 concentration of detected samples was determined to be 7.29 ng/mL with a range of 3.90 - 9.46 ng/mL.

  • Sandwich ELISA standard curve of MP02864-2

    Sandwich ELISA standard curve of MP02864-2

    Sandwich ELISA standard curve of MP02864-2, Human CD21 Recombinant Matched Antibody Pair - PBS only. 87097-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2980. 87097-1-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL

  • Affinity and Kinetic Characterization of 87097-1-RR

    Affinity and Kinetic Characterization of 87097-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87097-1-RR against Human CD21 were performed. The affinity constant is 5.60 nM.

CD21 Recombinant monoclonal antibody, PBS Only (Detector)

CD21 Uni-rAb® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 87097-1-PBS

产品说明书

CloneNo. 252181F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Sandwich ELISA, Indirect ELISA

别名

C3DR, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87097-1-PBS targets CD21 as part of a matched antibody pair:

MP02864-2: 87097-3-PBS capture and 87097-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2980

Product name: recombinant human CR2 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 21-971 aa of NM_001877.5

Sequence: ISCGSPPPILNGRISYYSTPIAVGTVIRYSCSGTFRLIGEKSLLCITKDKVDGTWDKPAPKCEYFNKYSSCPEPIVPGGYKIRGSTPYRHGDSVTFACKTNFSMNGNKSVWCQANNMWGPTRLPTCVSVFPLECPALPMIHNGHHTSENVGSIAPGLSVTYSCESGYLLVGEKIINCLSSGKWSAVPPTCEEARCKSLGRFPNGKVKEPPILRVGVTANFFCDEGYRLQGPPSSRCVIAGQGVAWTKMPVCEEIFCPSPPPILNGRHIGNSLANVSYGSIVTYTCDPDPEEGVNFILIGESTLRCTVDSQKTGTWSGPAPRCELSTSAVQCPHPQILRGRMVSGQKDRYTYNDTVIFACMFGFTLKGSKQIRCNAQGTWEPSAPVCEKECQAPPNILNGQKEDRHMVRFDPGTSIKYSCNPGYVLVGEESIQCTSEGVWTPPVPQCKVAACEATGRQLLTKPQHQFVRPDVNSSCGEGYKLSGSVYQECQGTIPWFMEIRLCKEITCPPPPVIYNGAHTGSSLEDFPYGTTVTYTCNPGPERGVEFSLIGESTIRCTSNDQERGTWSGPAPLCKLSLLAVQCSHVHIANGYKISGKEAPYFYNDTVTFKCYSGFTLKGSSQIRCKADNTWDPEIPVCEKETCQHVRQSLQELPAGSRVELVNTSCQDGYQLTGHAYQMCQDAENGIWFKKIPLCKVIHCHPPPVIVNGKHTGMMAENFLYGNEVSYECDQGFYLLGEKKLQCRSDSKGHGSWSGPSPQCLRSPPVTRCPNPEVKHGYKLNKTHSAYSHNDIVYVDCNPGFIMNGSRVIRCHTDNTWVPGVPTCIKKAFIGCPPPPKTPNGNHTGGNIARFSPGMSILYSCDQGYLLVGEALLLCTHEGTWSQPAPHCKEVNCSSPADMDGIQKGLEPRKMYQYGAVVTLECEDGYMLEGSPQSQCQSDHQWNPPLAVCRSR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complement component (3d/Epstein Barr virus) receptor 2
别名 C3DR, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor
计算分子量113 kDa
观测分子量150 kDa
GenBank蛋白编号NM_001877.5
基因名称 CD21
Gene ID (NCBI) 1380
ENSEMBL Gene IDENSG00000117322
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP20023-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

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