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验证数据展示

  • ChIP experiment of Raji using 86937-1-RR

    ChIP experiment of Raji using 86937-1-RR

    Chromatin was prepared from Raji cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of FOXP1 (86937-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  • WB analysis using 86937-1-RR

    WB analysis using 86937-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86937-1-RR (FOXP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86937-1-RR

    WB analysis using 86937-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86937-1-RR (FOXP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 86937-1-RR

    IF Staining of Jurkat using 86937-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using FOXP1 antibody (86937-1-RR, Clone: 243153D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86937-1-RR

    Affinity and Kinetic Characterization of 86937-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86937-1-RR against Human FOXP1 were performed. The affinity constant is 1.36 nM.

FOXP1 Recombinant monoclonal antibody

FOXP1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA, ChIP-qPCR
Cat No. 86937-1-RR

产品说明书

CloneNo. 243153D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA, ChIP-qPCR

别名

12CC4, forkhead box P1, Forkhead box protein P1, hFKH1B, HSPC215

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Jurkat cells
Positive IF/ICC detected inJurkat cells
Positive ChIP-qPCR detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy FOXP1 Ready-To-Use IHC Kit. FOXP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86937-1-RR targets FOXP1 in WB, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA, ChIP-qPCR Application Description
经测试反应性 human
免疫原

CatNo: Ag17045

Product name: Recombinant human FOXP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 349-457 aa of BC131720

Sequence: QLELQLAKDKERLQAMMTHLHVKSTEPKAAPQPLNLVSSVTLSKSASEASPQSLPHTPTTPTAPLTPVTQGPSVITTTSMHTVGPIRRRYSDKYNVPISSADIAQNQEF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box P1
别名 12CC4, forkhead box P1, Forkhead box protein P1, hFKH1B, HSPC215
计算分子量 677 aa, 75 kDa
观测分子量75-77 kDa, 65-67 kDa
GenBank蛋白编号BC131720
基因名称 FOXP1
Gene ID (NCBI) 27086
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H334
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXP1, also known as Mac-1-regulated forkhead, is a 677 amino acid protein, which forms homodimers and heterodimers with FOXP2 and FOXP4 (PubMed:25027557). Dimerization is required for DNA-binding. FOXP1 has an important function in neuronal development.9 Mutations of its gene, FOXP1, located on chromosome 3p14.1,7 can result in the development of autism spectrum disorder, intellectual disability, speech and language deficits as well as motor development delay. FOXP1 is also engaged in lung and esophagus morphogenesis, as well as in B-cell development.7,10 The widely researched role of FOXP1 in carcinogenesis is of great importance, although still unclear to some extent. FOXP1 exists some isoforms with MV 75-77 kDa, 65-67 kDa, 12 kDa.

实验方案

Product Specific Protocols
IF protocol for FOXP1 antibody 86937-1-RRDownload protocol
WB protocol for FOXP1 antibody 86937-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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