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验证数据展示

  • WB analysis using 86856-1-RR (same clone as 86856-1-PBS)

    WB analysis using 86856-1-RR (same clone as 86856-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86856-1-RR (POLA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86856-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86856-1-RR (same clone as 86856-1-PBS)

    IF Staining of HeLa using 86856-1-RR (same clone as 86856-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLA2 antibody (86856-1-RR, Clone: 251924A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86856-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02735-1

    Cytometric bead array sample test of MP02735-1

    The mean POLA2 concentration was determined to be 1.6 ng/mL in COLO 320 cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP02735-1

    Cytometric bead array standard curve of MP02735-1

    Cytometric bead array standard curve of MP02735-1, POLA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86856-1-PBS. Detection antibody: 86856-2-PBS. Standard: Ag0271. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 86856-1-RR

    Affinity and Kinetic Characterization of 86856-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86856-1-RR against Human POLA2 were performed. The affinity constant is below 1 pM.

POLA2 Recombinant monoclonal antibody, PBS Only (Capture)

POLA2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86856-1-PBS

产品说明书

CloneNo. 251924A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

DNA polymerase alpha subunit B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86856-1-PBS targets POLA2 as part of a matched antibody pair:

MP02735-1: 86856-1-PBS capture and 86856-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0271

Product name: Recombinant human POLA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 17-275 aa of BC001347

Sequence: LDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSEILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polymerase (DNA directed), alpha 2 (70kD subunit)
别名 DNA polymerase alpha subunit B
计算分子量 70 kDa
观测分子量68-70 kDa
GenBank蛋白编号BC001347
基因名称 POLA2
Gene ID (NCBI) 23649
RRIDAB_3745166
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14181
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POLA2 (DNA polymerase α subunit B) is the non-catalytic regulatory subunit of DNA polymerase α (Polα-primase complex). It connects the catalytic subunit POLA1 to the primase subunits PRIM1/PRIM2, stabilizing the tetrameric complex and ensuring primer synthesis and translocation to the polymerase active site to initiate DNA replication. This protein is expressed in the cell nucleus and is crucial for DNA replication and cell cycle progression in eukaryotes.

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