验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, COLO 320 cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells, PC-12 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86856-1-RR targets POLA2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag0271 Product name: Recombinant human POLA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 17-275 aa of BC001347 Sequence: LDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSEILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | polymerase (DNA directed), alpha 2 (70kD subunit) |
| 别名 | DNA polymerase alpha subunit B |
| 计算分子量 | 70 kDa |
| 观测分子量 | 68-70 kDa |
| GenBank蛋白编号 | BC001347 |
| 基因名称 | POLA2 |
| Gene ID (NCBI) | 23649 |
| RRID | AB_3745166 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14181 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POLA2 (DNA polymerase α subunit B) is the non-catalytic regulatory subunit of DNA polymerase α (Polα-primase complex). It connects the catalytic subunit POLA1 to the primase subunits PRIM1/PRIM2, stabilizing the tetrameric complex and ensuring primer synthesis and translocation to the polymerase active site to initiate DNA replication. This protein is expressed in the cell nucleus and is crucial for DNA replication and cell cycle progression in eukaryotes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for POLA2 antibody 86856-1-RR | Download protocol |
| WB protocol for POLA2 antibody 86856-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
