×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86693-2-RR (same clone as 86693-2-PBS)

    WB analysis using 86693-2-RR (same clone as 86693-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86693-2-RR (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86693-2-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 86693-2-RR (same clone as 86693-2-PBS)

    WB analysis of HepG2 using 86693-2-RR (same clone as 86693-2-PBS)

    WB result of NQO1 antibody (86693-2-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NQO1 transfected HepG2 cells. This data was developed using the same antibody clone with 86693-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02801-1

    Sandwich ELISA sample test of MP02801-1

    The mean NQO1 concentration was determined to be 87.24 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load and 66.51 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02801-1

    Sandwich ELISA standard curve of MP02801-1

    Sandwich ELISA standard curve of MP02801-1, Human NQO1 Recombinant Matched Antibody Pair - PBS only. 86693-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2009. 86693-2-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL

  • Affinity and Kinetic Characterization of 86693-2-RR

    Affinity and Kinetic Characterization of 86693-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86693-2-RR against Human NQO1 were performed. The affinity constant is 1.80 nM.

NQO1 Recombinant monoclonal antibody, PBS Only (Detector)

NQO1 Uni-rAb® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 86693-2-PBS

产品说明书

CloneNo. 251713C3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Sandwich ELISA, Indirect ELISA

别名

Azoreductase, DHQU, DIA 4, DIA4, DT diaphorase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

86693-2-PBS targets NQO1 as part of a matched antibody pair:

MP02801-1: 86693-1-PBS capture and 86693-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2009

Product name: Recombinant human NQO1 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-274 aa of BC007659

Sequence: MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NAD(P)H dehydrogenase, quinone 1
别名 Azoreductase, DHQU, DIA 4, DIA4, DT diaphorase
计算分子量 274 aa, 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC007659
基因名称 NQO1
Gene ID (NCBI) 1728
RRIDAB_3745057
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15559
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640, PMID:18848868)

||
New chat

Able™

正在加载，请稍候...