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验证数据展示

  • WB analysis using 86588-2-RR

    WB analysis using 86588-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86588-2-RR (FCGR2A+FCGR2B(CD32) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-937 using 86588-2-RR

    IF Staining of U-937 using 86588-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U-937 cells using CD32 antibody (86588-2-RR, Clone: 251087D9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 86588-2-RR

    Affinity of 86588-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86588-2-RR against Human CD32 were performed. The affinity constant is 5.25 nM.

CD32 Recombinant monoclonal antibody

CD32 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86588-2-RR

产品说明书

CloneNo. 251087D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CD32A, Fc gamma RII a, Fc gamma RIIa, FCG2, Fc-gamma RII-a

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-937 cells, K-562 cells
Positive IF/ICC detected inU-937 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy FCGR2A/CD32A Ready-To-Use IHC Kit. FCGR2A/CD32A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86588-2-RR targets CD32 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2713

Product name: Recombinant Human FCGR2A/CD32a protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 34-217 aa of NM_001136219.3

Sequence: QAAAPPKAVLKLEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPMG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIa, receptor (CD32)
别名 CD32A, Fc gamma RII a, Fc gamma RIIa, FCG2, Fc-gamma RII-a
计算分子量35 kDa
观测分子量40 kDa
GenBank蛋白编号NM_001136219.3
基因名称 CD32a
Gene ID (NCBI) 2212
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12318-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

实验方案

Product Specific Protocols
IF protocol for CD32 antibody 86588-2-RRDownload protocol
WB protocol for CD32 antibody 86588-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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