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验证数据展示

  • WB analysis using 86588-2-RR (same clone as 86588-2-PBS)

    WB analysis using 86588-2-RR (same clone as 86588-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86588-2-RR (FCGR2A+FCGR2B(CD32) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86588-2-PBS in a different storage buffer formulation.

  • IF Staining of U-937 using 86588-2-RR (same clone as 86588-2-PBS)

    IF Staining of U-937 using 86588-2-RR (same clone as 86588-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-937 cells using CD32 antibody (86588-2-RR, Clone: 251087D9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86588-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02532-1

    Cytometric bead array sample test of MP02532-1

    The mean FCGR2A / CD32a concentration was determined to be 360.3 ng/mL in K-562 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02532-1

    Cytometric bead array standard curve of MP02532-1

    Cytometric bead array standard curve of MP02532-1, FCGR2A / CD32a Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86588-2-PBS. Detection antibody: 86588-1-PBS. Standard: Eg2713. Range: 0.781-50 ng/mL.

  • Affinity of 86588-2-RR

    Affinity of 86588-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86588-2-RR against Human CD32 were performed. The affinity constant is 5.25 nM.

CD32 Recombinant monoclonal antibody, PBS Only (Capture)

CD32 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86588-2-PBS

产品说明书

CloneNo. 251087D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

CD32A, Fc gamma RII a, Fc gamma RIIa, FCG2, Fc-gamma RII-a

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FCGR2A/CD32A Ready-To-Use IHC Kit. FCGR2A/CD32A primary antibody included.

产品信息

86588-2-PBS targets CD32 as part of a matched antibody pair:

MP02532-1: 86588-2-PBS capture and 86588-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2713

Product name: Recombinant Human FCGR2A/CD32a protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 34-217 aa of NM_001136219.3

Sequence: QAAAPPKAVLKLEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPMG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIa, receptor (CD32)
别名 CD32A, Fc gamma RII a, Fc gamma RIIa, FCG2, Fc-gamma RII-a
计算分子量35 kDa
观测分子量40 kDa
GenBank蛋白编号NM_001136219.3
基因名称 CD32a
Gene ID (NCBI) 2212
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12318-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

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