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验证数据展示

  • Sandwich ELISA sample test of MP02481-1

    Sandwich ELISA sample test of MP02481-1

    The mean CD73 concentration was determined to be 67.84 ng/mL in U-87 MG cell extract based on a 1.5 mg/mL extract load and 125.52 ng/mL in PC-3 cell extract based on a 1.1 mg/mL extract load.

  • Sandwich ELISA sample test of MP02481-1

    Sandwich ELISA sample test of MP02481-1

    Plasma of six individual healthy human donors was measured. The human CD73 concentration of detected samples was determined to be 15.14 ng/mL with a range of 8.55 - 28.73 ng/mL.

  • Sandwich ELISA standard curve of MP02481-1

    Sandwich ELISA standard curve of MP02481-1

    Sandwich ELISA standard curve of MP02481-1, Human CD73 Recombinant Matched Antibody Pair - PBS only. 86512-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2974. 86512-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Cytometric bead array sample test of MP02481-1

    Cytometric bead array sample test of MP02481-1

    The mean CD73 concentration was determined to be 895.3 ng/mL in U-87 MG cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP02481-1

    Cytometric bead array standard curve of MP02481-1

    Cytometric bead array standard curve of MP02481-1, CD73 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86512-2-PBS. Detection antibody: 86512-1-PBS. Standard: Eg2974. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 86512-1-PBS

    Affinity and Kinetic Characterization of 86512-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 86512-1-PBS against Human NT5E was performed. The affinity constant is 1.63 nM.

NT5E Recombinant monoclonal antibody, PBS Only (Detector)

NT5E Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 86512-1-PBS

产品说明书

CloneNo. 251250E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

CD73, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86512-1-PBS targets NT5E as part of a matched antibody pair:

MP02481-1: 86512-2-PBS capture and 86512-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2974

Product name: Recombinant Human CD73 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 27-547 aa of NM_002526.3

Sequence: WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 5'-nucleotidase, ecto (CD73)
别名 CD73, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase
计算分子量63 kDa
GenBank蛋白编号NM_002526.3
基因名称 CD73
Gene ID (NCBI) 4907
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP21589-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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