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验证数据展示

  • WB analysis using 86236-1-RR

    WB analysis using 86236-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86236-1-RR (FOXO4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293T using 86236-1-RR

    IF Staining of HEK-293T using 86236-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using FOXO4 antibody (86236-1-RR, Clone: 250921F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86236-1-RR

    Affinity and Kinetic Characterization of 86236-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86236-1-RR against Human FOXO4 were performed. The affinity constant is 2.40 nM.

FOXO4 Recombinant monoclonal antibody

FOXO4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86236-1-RR

产品说明书

CloneNo. 250921F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

AFX, AFX1, MLLT7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293T cells, HuT 78 cells, Ramos cells, Jurkat cells, PC-12 cells, mouse heart tissue
Positive IF/ICC detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy FOXO4 Ready-To-Use IHC Kit. FOXO4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86236-1-RR targets FOXO4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16169

Product name: Recombinant human FOXO4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 198-505 aa of BC106761

Sequence: MDSSSKLLRGRSKAPKKKPSVLPAPPEGATPTSPVGHFAKWSGSPCSRNREEADMWTTFRPRSSSNASSVSTRLSPLRPESEVLAEEIPASVSSYAGGVPPTLNEGLELLDGLNLTSSHSLLSRSGLSGFSLQHPGVTGPLHTYSSSLFSPAEGPLSAGEGCFSSSQALEALLTSDTPPPPADVLMTQVDPILSQAPTLLLLGGLPSSSKLATGVGLCPKPLEAPGPSSLVPTLSMIAPPPVMASAPIPKALGTPVLTPPTEAASQDRMPQDLDLDMYMENLECDMDNIISDLMDEGEGLDFNFEPDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box O4
别名 AFX, AFX1, MLLT7
计算分子量 505 aa, 54 kDa
观测分子量 65 kDa
GenBank蛋白编号BC106761
基因名称 FOXO4
Gene ID (NCBI) 4303
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP98177
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor that is involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO downregulates the expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias.

实验方案

Product Specific Protocols
IF protocol for FOXO4 antibody 86236-1-RRDownload protocol
WB protocol for FOXO4 antibody 86236-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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