验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-86236 targets FOXO4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag16169 Product name: Recombinant human FOXO4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 198-505 aa of BC106761 Sequence: MDSSSKLLRGRSKAPKKKPSVLPAPPEGATPTSPVGHFAKWSGSPCSRNREEADMWTTFRPRSSSNASSVSTRLSPLRPESEVLAEEIPASVSSYAGGVPPTLNEGLELLDGLNLTSSHSLLSRSGLSGFSLQHPGVTGPLHTYSSSLFSPAEGPLSAGEGCFSSSQALEALLTSDTPPPPADVLMTQVDPILSQAPTLLLLGGLPSSSKLATGVGLCPKPLEAPGPSSLVPTLSMIAPPPVMASAPIPKALGTPVLTPPTEAASQDRMPQDLDLDMYMENLECDMDNIISDLMDEGEGLDFNFEPDP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | forkhead box O4 |
| 别名 | AFX, AFX1, MLLT7 |
| 计算分子量 | 505 aa, 54 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC106761 |
| 基因名称 | FOXO4 |
| Gene ID (NCBI) | 4303 |
| RRID | AB_3747826 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P98177 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor that is involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO downregulates the expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FOXO4 antibody CL488-86236 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
