验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, U2Os cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85907-4-RR targets GRK6 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | GRK6 fusion protein Ag1992 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor kinase 6 |
| 别名 | EC:2.7.11.16, G protein-coupled receptor kinase 6, G protein-coupled receptor kinase GRK6, GPRK6 |
| 计算分子量 | 66 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC017272 |
| 基因名称 | GRK6 |
| Gene ID (NCBI) | 2870 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P43250 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GRK6 (G Protein-Coupled Receptor Kinase 6) is a member of the G protein-coupled receptor kinase (GRK) family, which plays a crucial role in regulating the activity of G protein-coupled receptors (GPCRs). GRK6 specifically phosphorylates the activated forms of GPCRs, initiating their desensitization, internalization, and signaling events that lead to receptor deactivation. This process is essential for maintaining proper cellular responses to extracellular signals.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GRK6 antibody 85907-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |