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验证数据展示

  • WB analysis of rat kidney using 85870-4-RR (same clone as 85870-4-PBS)

    WB analysis of rat kidney using 85870-4-RR (same clone as 85870-4-PBS)

    Rat kidney tissue lysates were subjected to SDS PAGE followed by western blot with 85870-4-RR (ARIP2; SYNJ2BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85870-4-PBS in a different storage buffer formulation.

  • WB analysis using 85870-4-RR (same clone as 85870-4-PBS)

    WB analysis using 85870-4-RR (same clone as 85870-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85870-4-RR (ARIP2; SYNJ2BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85870-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85870-4-RR (same clone as 85870-4-PBS)

    IF Staining of HeLa using 85870-4-RR (same clone as 85870-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARIP2; SYNJ2BP antibody (85870-4-RR, Clone: 250205D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85870-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP02180-1

    Cytometric bead array standard curve of MP02180-1

    Cytometric bead array standard curve of MP02180-1, SYNJ2BP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85870-4-PBS. Detection antibody: 85870-3-PBS. Standard: Ag8130. Range: 0.781-25 ng/mL.

  • Affinity and Kinetic Characterization of 85870-4-RR

    Affinity and Kinetic Characterization of 85870-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85870-4-RR against Human SYNJ2BP were performed. The affinity constant is 0.68 nM.

SYNJ2BP Recombinant monoclonal antibody, PBS Only (Capture)

SYNJ2BP Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 85870-4-PBS

产品说明书

CloneNo. 250205D7

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ARIP2, OMP25, Synaptojanin-2-binding protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85870-4-PBS targets SYNJ2BP as part of a matched antibody pair:

MP02180-1: 85870-4-PBS capture and 85870-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag8130

Product name: Recombinant human ARIP2; SYNJ2BP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-112 aa of BC007704

Sequence: MNGRVDYLVTEEEINLTRGPSGLGFNIVGGTDQQYVSNDSGIYVSRIKENGAAALDGRLQEGDKILSVNGQDLKNLLHQDAVDLFRNAGYAVSLRVQHRLQVQNGPIGHRGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptojanin 2 binding protein
别名 ARIP2, OMP25, Synaptojanin-2-binding protein
计算分子量 145 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC007704
基因名称 SYNJ2BP
Gene ID (NCBI) 55333
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP57105
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. SYNJ2BP is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. SYNJ2BP may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. A double band of size 15.9 kDa can be detected in Western blot (PMID: 23284606).

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