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验证数据展示

  • WB analysis of rat kidney using 85870-4-RR

    WB analysis of rat kidney using 85870-4-RR

    Rat kidney tissue lysates were subjected to SDS PAGE followed by western blot with 85870-4-RR (ARIP2; SYNJ2BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85870-4-RR

    WB analysis using 85870-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85870-4-RR (ARIP2; SYNJ2BP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85870-4-RR

    IF Staining of HeLa using 85870-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARIP2; SYNJ2BP antibody (85870-4-RR, Clone: 250205D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SYNJ2BP Recombinant antibody

SYNJ2BP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85870-4-RR

产品说明书

CloneNo. 250205D7

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

ARIP2, OMP25, Synaptojanin-2-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, rat kidney tissue, SGC-7901 cells, SH-SY5Y cells, human heart tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85870-4-RR targets SYNJ2BP in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 SYNJ2BP fusion protein Ag8130 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptojanin 2 binding protein
别名 ARIP2, OMP25, Synaptojanin-2-binding protein
计算分子量 145 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC007704
基因名称 SYNJ2BP
Gene ID (NCBI) 55333
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP57105
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. SYNJ2BP is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. SYNJ2BP may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. A double band of size 15.9 kDa can be detected in Western blot (PMID: 23284606).

实验方案

Product Specific Protocols
WB protocol for SYNJ2BP antibody 85870-4-RRDownload protocol
IF protocol for SYNJ2BP antibody 85870-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols