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Host
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 85640-4-RR (same clone as 85640-4-PBS)

    WB analysis using 85640-4-RR (same clone as 85640-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85640-4-RR (SF3B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85640-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP02058-3

    Sandwich ELISA standard curve of MP02058-3

    Sandwich ELISA standard curve of MP02058-3, Human SF3B1 Recombinant Matched Antibody Pair - PBS only. 85640-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26689. 85640-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP02058-3

    Sample test of MP02058-3

    The mean SF3B1 concentration was determined to be 32.11 ng/mL in MCF-7 cell extract based on a 1.3 mg/mL extract load and 48.40 ng/mL in HeLa cell extract based on a 1.8 mg/mL extract load.

SF3B1 Recombinant antibody, PBS Only (Detector)

SF3B1 Uni-rAbTM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85640-4-PBS

产品说明书

CloneNo. 243145C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Sandwich ELISA, Indirect ELISA, Sample test

别名

PRP10, SAP 155, SAP155, SF3b155

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SF3B1 Ready-To-Use IHC Kit. SF3B1 primary antibody included.

产品信息

85640-4-PBS targets SF3B1 as part of a matched antibody pair:

MP02058-3: 85640-3-PBS capture and 85640-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 SF3B1 fusion protein Ag26689 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 splicing factor 3b, subunit 1, 155kDa
别名 PRP10, SAP 155, SAP155, SF3b155
计算分子量 146 kDa
观测分子量146 kDa
GenBank蛋白编号NM_012433
基因名称 SF3B1
Gene ID (NCBI) 23451
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75533
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.