IHCeasy SF3B1 Ready-To-Use IHC Kit

SF3B1 Ready-to-use reagent kit for IHC.

Reactivity

Human

Sample Type

FFPE tissue

Cat No : KHC1077

Synonyms

PRP10, PRPF10, SAP 155, SAP155, SF3B1, SF3b155, Splicing factor 3B subunit 1



Product Information

KHC1077 is a ready-to-use IHC kit for staining of SF3B1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy SF3B1 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms PRP10, PRPF10, SAP 155, SAP155, SF3B1, SF3b155, Splicing factor 3B subunit 1