验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, unboiled mouse kidney tissue |
Planning an IHC experiment? We recommend our IHCeasy AQP2 Ready-To-Use IHC Kit. AQP2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85402-1-RR targets AQP2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | AQP2 fusion protein Ag29931 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | aquaporin 2 (collecting duct) |
| 别名 | Aquaporin 2, AQP-2, Aquaporin2 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 28-29, 35-38 kDa |
| GenBank蛋白编号 | NM_000486 |
| 基因名称 | AQP2 |
| Gene ID (NCBI) | 359 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P41181 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AQP2 (Aquaporin2) is a water channel protein that is widely distributed among mammalian tissues and plays a major role in water homeostasis. AQP2 is mainly found in the apical cell membranes of the kidney's collecting duct cells and is critical for urinary concentration. AQP2 can be glycosylated and this antibody recognizes the 28-29 kDa non-glycosylated as well as the 35-40 kDa glycosylated forms of AQP2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AQP2 antibody 85402-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |