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验证数据展示

  • WB analysis using 85054-2-RR (same clone as 85054-2-PBS)

    WB analysis using 85054-2-RR (same clone as 85054-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85054-2-RR (ADNP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85054-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85054-2-RR (same clone as 85054-2-PBS)

    IF Staining of HeLa using 85054-2-RR (same clone as 85054-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ADNP antibody (85054-2-RR, Clone: 242687C11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85054-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 85054-2-RR (same clone as 85054-2-PBS)

    FC experiment of HeLa using 85054-2-RR (same clone as 85054-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ADNP Recombinant antibody (85054-2-RR, Clone:242687C11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85054-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01783-2

    Cytometric bead array standard curve of MP01783-2

    Cytometric bead array standard curve of MP01783-2, ADNP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85054-2-PBS. Detection antibody: 85054-1-PBS. Standard: Ag12414. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01783-1

    Cytometric bead array standard curve of MP01783-1

    Cytometric bead array standard curve of MP01783-1, ADNP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85054-2-PBS. Detection antibody: 85054-3-PBS. Standard: Ag12414. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 85054-2-RR

    Affinity and Kinetic Characterization of 85054-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85054-2-RR against Human ADNP were performed. The affinity constant is below 1 pM.

ADNP Recombinant antibody, PBS Only (Capture)

ADNP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 85054-2-PBS

产品说明书

CloneNo. 242687C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

Activity-dependent neuroprotective protein, Activity-dependent neuroprotector homeobox protein, ADNP1, KIAA0784

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85054-2-PBS targets ADNP as part of a matched antibody pair:

MP01783-1: 85054-2-PBS capture and 85054-3-PBS detection (validated in Cytometric bead array)

MP01783-2: 85054-2-PBS capture and 85054-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 ADNP fusion protein Ag12414 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 activity-dependent neuroprotector homeobox
别名 Activity-dependent neuroprotective protein, Activity-dependent neuroprotector homeobox protein, ADNP1, KIAA0784
计算分子量 1102 aa, 123 kDa
观测分子量 135-145 kDa
GenBank蛋白编号BC075794
基因名称 ADNP
Gene ID (NCBI) 23394
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H2P0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C