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验证数据展示

  • WB analysis using 85054-2-RR

    WB analysis using 85054-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85054-2-RR (ADNP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85054-2-RR

    IF Staining of HeLa using 85054-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ADNP antibody (85054-2-RR, Clone: 242687C11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 85054-2-RR

    FC experiment of HeLa using 85054-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ADNP Recombinant antibody (85054-2-RR, Clone:242687C11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85054-2-RR

    Affinity and Kinetic Characterization of 85054-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85054-2-RR against Human ADNP were performed. The affinity constant is below 1 pM.

ADNP Recombinant monoclonal antibody

ADNP Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85054-2-RR

产品说明书

CloneNo. 242687C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Activity-dependent neuroprotective protein, Activity-dependent neuroprotector homeobox protein, ADNP1, KIAA0784

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, SH-SY5Y cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85054-2-RR targets ADNP in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12414

Product name: Recombinant human ADNP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 757-1102 aa of BC075794

Sequence: KGHEDDSYEARKSFLTKYFNKQPYPTRREIEKLAASLWLWKSDIASHFSNKRKKCVRDCEKYKPGVLLGFNMKELNKVKHEMDFDAEWLFENHDEKDSRVNASKTADKKLNLGKEDDSSSDSFENLEEESNESGSPFDPVFEVEPKISNDNPEEHVLKVIPEDASESEEKLDQKEDGSKYETIHLTEEPTKLMHNASDSEVDQDDVVEWKDGASPSESGPGSQQVSDFEDNTCEMKPGTWSDESSQSEDARSSKPAAKKKATMQGDREQLKWKNSSYGKVEGFWSKDQSQWKNASENDERLSNPQIEWQNSTIDSEDGEQFDNMTDGVAEPMHGSLAGVKLSSQQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 activity-dependent neuroprotector homeobox
别名 Activity-dependent neuroprotective protein, Activity-dependent neuroprotector homeobox protein, ADNP1, KIAA0784
计算分子量 1102 aa, 123 kDa
观测分子量 135-145 kDa
GenBank蛋白编号BC075794
基因名称 ADNP
Gene ID (NCBI) 23394
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H2P0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for ADNP antibody 85054-2-RRDownload protocol
WB protocol for ADNP antibody 85054-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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