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验证数据展示

  • IHC staining of mouse brain using 84875-5-RR

    IHC staining of mouse brain using 84875-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84875-5-RR (MAPK10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK10 antibody included
  • IHC staining of mouse brain using 84875-5-RR

    IHC staining of mouse brain using 84875-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84875-5-RR (MAPK10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAPK10 antibody included
  • IF Staining of HepG2 using 84875-5-RR

    IF Staining of HepG2 using 84875-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using JNK antibody (84875-5-RR, Clone: 242364A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84875-5-RR

    FC experiment of HeLa using 84875-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAPK10 Recombinant antibody (84875-5-RR, Clone:242364A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84875-5-RR

    Affinity and Kinetic Characterization of 84875-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84875-5-RR against Human MAPK10 were performed. The affinity constant is 2.45 nM.

JNK Recombinant monoclonal antibody

JNK Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84875-5-RR

产品说明书

CloneNo. 242364A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

MAPK10, 242364A10, c-Jun N-terminal kinase 3, EC:2.7.11.24, JNK1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAPK10 Ready-To-Use IHC Kit. MAPK10 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84875-5-RR targets JNK in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11785

Product name: Recombinant human MAPK10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-319 aa of BC065516

Sequence: MELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMVRHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAKRISVDDALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHTIEEWKELIYKEVMNSEEKTKNGVVKGQPSPSGAAVNSSESLPPSSSVNDISSMSTDQTLASDTDSSLEASAGPLGCCR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase 10
别名 MAPK10, 242364A10, c-Jun N-terminal kinase 3, EC:2.7.11.24, JNK1
计算分子量 464 aa, 53 kDa
GenBank蛋白编号BC065516
基因名称 JNK3
Gene ID (NCBI) 5602
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP53779
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for JNK antibody 84875-5-RRDownload protocol
IF protocol for JNK antibody 84875-5-RRDownload protocol
IHC protocol for JNK antibody 84875-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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