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验证数据展示

  • WB analysis using 84869-1-RR (same clone as 84869-1-PBS)

    WB analysis using 84869-1-RR (same clone as 84869-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84869-1-RR (PDE10A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84869-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84869-1-RR (same clone as 84869-1-PBS)

    FC experiment of HeLa using 84869-1-RR (same clone as 84869-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDE10A Recombinant antibody (84869-1-RR, Clone:242357E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84869-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP01645-2

    Sandwich ELISA sample test of MP01645-2

    The mean PDE10A concentration was determined to be 62.34 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load, 60.49 ng/mL in Neuro-2a cell extract based on a 1.50 mg/mL extract load and 71.70 ng/mL in SH-SY5Y cell extract based on a 1.00 mg/mL extract load.

  • Sandwich ELISA standard curve of MP01645-2

    Sandwich ELISA standard curve of MP01645-2

    Sandwich ELISA standard curve of MP01645-2, Human PDE10A Recombinant Matched Antibody Pair - PBS only. 84869-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12771. 84869-1-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • Cytometric bead array standard curve of MP01645-2

    Cytometric bead array standard curve of MP01645-2

    Cytometric bead array standard curve of MP01645-2, PDE10A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84869-2-PBS. Detection antibody: 84869-1-PBS. Standard: Ag12771. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84869-1-RR

    Affinity and Kinetic Characterization of 84869-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84869-1-RR against Human PDE10A were performed. The affinity constant is 3.37 nM.

PDE10A Recombinant monoclonal antibody, PBS Only (Detector)

PDE10A Uni-rAb® Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 84869-1-PBS

产品说明书

CloneNo. 242357E12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

242357E12, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A, EC:3.1.4.17, HSPDE10A, phosphodiesterase 10A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® PDE10A Ready-To-Use IHC Kit. PDE10A primary antibody included.

产品信息

84869-1-PBS targets PDE10A as part of a matched antibody pair:

MP01645-2: 84869-2-PBS capture and 84869-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12771

Product name: Recombinant human PDE10A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 434-779 aa of BC104860

Sequence: KLSYHSICTSEEWQGLMQFTLPVRLCKEIELFHFDIGPFENMWPGIFVYMVHRSCGTSCFELEKLCRFIMSVKKNYRRVPYHNWKHAVTVAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGFSNSYLQKFDHPLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAIIATDLALYFGNRKQLEEMYQTGSLNLNNQSHRDRVIGLMMTACDLCSVTKLWPVTKLTANDIYAEFWAEGDEMKKLGIQPIPMMDRDKKDEVPQGQLGFYNAVAIPCYTTLTQILPPTEPLLKACRDNLSQWEKVIRGEETATWISSPSVAQKAAASED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 phosphodiesterase 10A
别名 242357E12, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A, EC:3.1.4.17, HSPDE10A, phosphodiesterase 10A
计算分子量 779 aa, 88 kDa
观测分子量80 kDa
GenBank蛋白编号BC104860
基因名称 PDE10A
Gene ID (NCBI) 10846
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y233
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDE10A (Phosphodiesterase 10A) is a single member of the PDE10 protein family, and it catalyzes both cAMP and cGMP hydrolysis (PMID: 34550322). The literature on PDE10A has been focused on psychiatric and neurological disorders because PDE10A expression is enriched in the striatal region of the brain under normal conditions (PMID: 12967715). Moreover, PDE10A expression is induced in various human tumor cell lines and PDE10A inhibition suppresses tumor cell growth (PMID: 37232184).

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