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验证数据展示

  • WB analysis using 84217-4-RR (same clone as 84217-4-PBS)

    WB analysis using 84217-4-RR (same clone as 84217-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84217-4-RR (KLHDC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84217-4-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 84217-4-RR (same clone as 84217-4-PBS)

    FC experiment of PC-3 using 84217-4-RR (same clone as 84217-4-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug KLHDC3 Recombinant antibody (84217-4-RR, Clone:241475G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84217-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01151-2

    Cytometric bead array standard curve of MP01151-2

    Cytometric bead array standard curve of MP01151-2, KLHDC3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84217-4-PBS. Detection antibody: 84217-2-PBS. Standard: Ag4879. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01151-3

    Cytometric bead array standard curve of MP01151-3

    Cytometric bead array standard curve of MP01151-3, KLHDC3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84217-4-PBS. Detection antibody: 84217-1-PBS. Standard: Ag4879. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84217-4-RR

    Affinity and Kinetic Characterization of 84217-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84217-4-RR against Human KLHDC3 were performed. The affinity constant is below 1 pM.

KLHDC3 Recombinant antibody, PBS Only (Capture)

KLHDC3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84217-4-PBS

产品说明书

CloneNo. 241475G5

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

PEAS, Kelch domain-containing protein 3, hPEAS, 241475G5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84217-4-PBS targets KLHDC3 as part of a matched antibody pair:

MP01151-2: 84217-4-PBS capture and 84217-2-PBS detection (validated in Cytometric bead array)

MP01151-3: 84217-4-PBS capture and 84217-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 KLHDC3 fusion protein Ag4879 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kelch domain containing 3
别名 PEAS, Kelch domain-containing protein 3, hPEAS, 241475G5
计算分子量 382 aa, 43 kDa
观测分子量43 kDa
GenBank蛋白编号BC041793
基因名称 KLHDC3
Gene ID (NCBI) 116138
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BQ90
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Kelch domain-containing protein 3 (KLHDC3), also designated PEAS, contains 5 Kelch repeats and may be involved in meiotic recombination process. The gene encoding KLHDC3 maps to chromosome 6, which contains around 1,200 genes within 170 million base pairs of sequence. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer suggesting the presence of a cancer susceptibility locus. Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome, 21-hydroxylase deficiency and maple syrup urine disease are also associated with genes on chromosome 6. KLHDC3 protein is mainly expressed in cytoplasm, also found in meiotic chromatin.