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验证数据展示

  • WB analysis using 84217-4-RR

    WB analysis using 84217-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84217-4-RR (KLHDC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-3 using 84217-4-RR

    FC experiment of PC-3 using 84217-4-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug KLHDC3 Recombinant antibody (84217-4-RR, Clone:241475G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84217-4-RR

    Affinity and Kinetic Characterization of 84217-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84217-4-RR against Human KLHDC3 were performed. The affinity constant is below 1 pM.

KLHDC3 Recombinant antibody

KLHDC3 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84217-4-RR

产品说明书

CloneNo. 241475G5

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

PEAS, Kelch domain-containing protein 3, hPEAS, 241475G5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCI-H1299 cells, PC-3 cells, HepG2 cells, K-562 cells, HEK-293T cells, rat testis tissue
Positive FC (Intra) detected inPC-3 cells, U-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84217-4-RR targets KLHDC3 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 KLHDC3 fusion protein Ag4879 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kelch domain containing 3
别名 PEAS, Kelch domain-containing protein 3, hPEAS, 241475G5
计算分子量 382 aa, 43 kDa
观测分子量43 kDa
GenBank蛋白编号BC041793
基因名称 KLHDC3
Gene ID (NCBI) 116138
RRIDAB_3671773
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BQ90
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Kelch domain-containing protein 3 (KLHDC3), also designated PEAS, contains 5 Kelch repeats and may be involved in meiotic recombination process. The gene encoding KLHDC3 maps to chromosome 6, which contains around 1,200 genes within 170 million base pairs of sequence. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer suggesting the presence of a cancer susceptibility locus. Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome, 21-hydroxylase deficiency and maple syrup urine disease are also associated with genes on chromosome 6. KLHDC3 protein is mainly expressed in cytoplasm, also found in meiotic chromatin.

实验方案

Product Specific Protocols
WB protocol for KLHDC3 antibody 84217-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols