• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84216-1-RR (same clone as 84216-1-PBS)

    WB analysis using 84216-1-RR (same clone as 84216-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84216-1-RR (MIER2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84216-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 84216-1-RR (same clone as 84216-1-PBS)

    IF Staining of A431 using 84216-1-RR (same clone as 84216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MIER2 antibody (84216-1-RR, Clone: 241458D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84216-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01148-1

    Cytometric bead array standard curve of MP01148-1

    Cytometric bead array standard curve of MP01148-1, MIER2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84216-2-PBS. Detection antibody: 84216-1-PBS. Standard: Ag11491. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01148-2

    Cytometric bead array standard curve of MP01148-2

    Cytometric bead array standard curve of MP01148-2, MIER2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84216-3-PBS. Detection antibody: 84216-1-PBS. Standard: Ag11491. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84216-1-RR

    Affinity and Kinetic Characterization of 84216-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84216-1-RR against Human MIER2 were performed. The affinity constant is below 1 pM.

MIER2 Recombinant antibody, PBS Only (Detector)

MIER2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84216-1-PBS

产品说明书

CloneNo. 241458D2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Mi-er2, Mesoderm induction early response protein 2, KIAA1193, 241458D2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84216-1-PBS targets MIER2 as part of a matched antibody pair:

MP01148-1: 84216-2-PBS capture and 84216-1-PBS detection (validated in Cytometric bead array)

MP01148-2: 84216-3-PBS capture and 84216-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 MIER2 fusion protein Ag11491 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mesoderm induction early response 1, family member 2
别名 Mi-er2, Mesoderm induction early response protein 2, KIAA1193, 241458D2
计算分子量 545 aa, 60 kDa
观测分子量60 kDa
GenBank蛋白编号BC028203
基因名称 MIER2
Gene ID (NCBI) 54531
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N344
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mesoderm induction early response 2 (MIER2) is a member of the MIER family of proteins, which is relatively conservative in evolution and includes three members: MIER1, MIER2, and MIER3. The MIER family is known to contribute to histone deacetylase (HDAC) activity and actively participate in histone deacetylation (PMID: 28046085; PMID: 38702028). In renal cell carcinoma, MIER2 represses PGC1A expression by recruiting HDAC1 to deacetylate P53 (PMID: 28046085).