验证数据展示
经过测试的应用
Positive WB detected in | U2OS cells, HEK-293 cells, Jurkat cells |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84216-1-RR targets MIER2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human |
免疫原 | MIER2 fusion protein Ag11491 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | mesoderm induction early response 1, family member 2 |
别名 | Mi-er2, Mesoderm induction early response protein 2, KIAA1193, 241458D2 |
计算分子量 | 545 aa, 60 kDa |
观测分子量 | 60 kDa |
GenBank蛋白编号 | BC028203 |
基因名称 | MIER2 |
Gene ID (NCBI) | 54531 |
RRID | AB_3671772 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q8N344 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mesoderm induction early response 2 (MIER2) is a member of the MIER family of proteins, which is relatively conservative in evolution and includes three members: MIER1, MIER2, and MIER3. The MIER family is known to contribute to histone deacetylase (HDAC) activity and actively participate in histone deacetylation (PMID: 28046085; PMID: 38702028). In renal cell carcinoma, MIER2 represses PGC1A expression by recruiting HDAC1 to deacetylate P53 (PMID: 28046085).
实验方案
Product Specific Protocols | |
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WB protocol for MIER2 antibody 84216-1-RR | Download protocol |
IF protocol for MIER2 antibody 84216-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |