验证数据展示
产品信息
83342-1-PBS targets RHOBTB1 as part of a matched antibody pair:
MP00383-1: 83342-2-PBS capture and 83342-1-PBS detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| 经测试应用 | WB, Indirect ELISA, Cytometric bead array Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag3365 Product name: Recombinant human RHOBTB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-344 aa of BC032848 Sequence: MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKSMWYPEIKHFCPRTPVILVGCQLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKELGLPYYETSVFDQFGIKDVFDNAIRAALISRRHLQFWKSHLKKVQKPLLQAPFLPPKAPPPVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFAHRIYLATSSSKFYDLFLMECEESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQAD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Rho-related BTB domain containing 1 |
| 别名 | KIAA0740, RHOBTB1 |
| 计算分子量 | 696 aa, 79 kDa |
| GenBank蛋白编号 | BC032848 |
| 基因名称 | RHOBTB1 |
| Gene ID (NCBI) | 9886 |
| RRID | AB_3671003 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O94844 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Rho-related BTB domain containing protein 1 (RhoBTB1) is also named as KIAA0740. RhoBTB1 is an atypical Rho GTPase with two BTB domains in addition to its Rho domain. RhoBTB1 is a substrate for ROCK1, and mutation of putative phosphorylation sites reduces its association with Cullin3, a scaffold for ubiquitin ligases (PMID: 31431478). RHOBTB1 is a novel PPARγ gene target in vascular smooth muscle cells that mediates the protective effect of PPARγ by serving as a substrate adaptor between the Cullin-3 RING ubiquitin ligase and phosphodiesterase 5, thus restraining its activity through ubiquitination and proteasomal degradation (PMID: 31789920).
