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验证数据展示

  • IF Staining of mouse brain using 83238-3-RR

    IF Staining of mouse brain using 83238-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOX8 antibody (83238-3-RR, Clone: 240120H6 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 83238-3-RR

    IF Staining of HeLa using 83238-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOX8 antibody (83238-3-RR, Clone: 240120H6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83238-3-RR

    Affinity and Kinetic Characterization of 83238-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83238-3-RR against Human SOX8 were performed. The affinity constant is 1.98 nM.

SOX8 Recombinant monoclonal antibody

SOX8 Uni-rAb® Recombinant Antibody for IF/ICC, IF-P, ELISA
Cat No. 83238-3-RR

产品说明书

CloneNo. 240120H6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IF-P, ELISA

别名

Transcription factor SOX-8, Transcription factor SOX 8, 240120H6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83238-3-RR targets SOX8 in IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34395

Product name: Recombinant human SOX8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 298-446 aa of BC031797

Sequence: EPGQAYGGAYFHAGASPVWAHKSAPSASASPTETGPPRPHIKTEQPSPGHYGDQPRGSPDYGSCSGQSSATPAAPAGPFAGSQGDYGDLQASSYYGAYPGYAPGLYQYPCFHSPRRPYASPLLNGLALPPAHSPTSHWDQPVYTTLTRP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SRY (sex determining region Y)-box 8
别名 Transcription factor SOX-8, Transcription factor SOX 8, 240120H6
计算分子量 446 aa, 47 kDa
GenBank蛋白编号BC031797
基因名称 SOX8
Gene ID (NCBI) 30812
RRIDAB_3670914
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP57073
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SOX8 antibody 83238-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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