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验证数据展示

  • IF Staining of mouse brain using 83238-3-RR (same clone as 83238-3-PBS)

    IF Staining of mouse brain using 83238-3-RR (same clone as 83238-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOX8 antibody (83238-3-RR, Clone: 240120H6 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83238-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83238-3-RR (same clone as 83238-3-PBS)

    IF Staining of HeLa using 83238-3-RR (same clone as 83238-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOX8 antibody (83238-3-RR, Clone: 240120H6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83238-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00222-1

    Cytometric bead array standard curve of MP00222-1

    Cytometric bead array standard curve of MP00222-1, SOX8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83238-1-PBS. Detection antibody: 83238-3-PBS. Standard: Ag34395. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00222-2

    Cytometric bead array standard curve of MP00222-2

    Cytometric bead array standard curve of MP00222-2, SOX8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83238-3-PBS. Detection antibody: 83238-2-PBS. Standard: Ag34395. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83238-3-RR

    Affinity and Kinetic Characterization of 83238-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83238-3-RR against Human SOX8 were performed. The affinity constant is 1.98 nM.

SOX8 Recombinant monoclonal antibody, PBS Only (Capture/Detector)

SOX8 Uni-rAb® Recombinant Antibody for IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 83238-3-PBS

产品说明书

CloneNo. 240120H6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

别名

240120H6, Transcription factor SOX 8, Transcription factor SOX-8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83238-3-PBS targets SOX8 as part of a matched antibody pair:

MP00222-1: 83238-1-PBS capture and 83238-3-PBS detection (validated in Cytometric bead array)

MP00222-2: 83238-3-PBS capture and 83238-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34395

Product name: Recombinant human SOX8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 298-446 aa of BC031797

Sequence: EPGQAYGGAYFHAGASPVWAHKSAPSASASPTETGPPRPHIKTEQPSPGHYGDQPRGSPDYGSCSGQSSATPAAPAGPFAGSQGDYGDLQASSYYGAYPGYAPGLYQYPCFHSPRRPYASPLLNGLALPPAHSPTSHWDQPVYTTLTRP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SRY (sex determining region Y)-box 8
别名 240120H6, Transcription factor SOX 8, Transcription factor SOX-8
计算分子量 446 aa, 47 kDa
GenBank蛋白编号BC031797
基因名称 SOX8
Gene ID (NCBI) 30812
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP57073
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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