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验证数据展示

  • WB analysis of HT-1080 using 83022-1-RR

    WB analysis of HT-1080 using 83022-1-RR

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 83022-1-RR (TRPV2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of K562 using 83022-1-RR

    FC experiment of K562 using 83022-1-RR

    1x10^6 K562 were intracellularly stained with 0.2 ug TRPV2 Recombinant antibody (83022-1-RR, Clone:230141C3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of SH-SY5Y using 83022-1-RR

    FC experiment of SH-SY5Y using 83022-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug TRPV2 Recombinant antibody (83022-1-RR, Clone:230141C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83022-1-RR

    Affinity and Kinetic Characterization of 83022-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83022-1-RR against Human TRPV2 were performed. The affinity constant is 31.3 pM.

TRPV2 Recombinant monoclonal antibody

TRPV2 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83022-1-RR

产品说明书

CloneNo. 230141C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

230141C3, Osm 9 like TRP channel 2, Osm-9-like TRP channel 2, OTRPC2, Vanilloid receptor-like protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-1080 cells
Positive FC (Intra) detected inK562, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83022-1-RR targets TRPV2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8864

Product name: Recombinant human TRPV2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC018926

Sequence: MTSPSSSPVFRLETLDGGQEDGSEADRGKLDFGSGLPPMESQFQGEDRKFAPQIRVNLNYRKGTGASQPDPNRFDRDRLFNAVSRGVPEDLAGLPEYLSKTSKYLTDSEYTEGSTGKTCLMKAVLNLKDGVNACILPLLQIDRDSGNPQPLVNAQCTDDYYRGHSALHIAIEKRSLQCVKLLVENGANVHARACGRFFQKGQGTCFYFGELPLSLAACTKQWDVVSYLLENPHQPASLQATDSQGNTVLHALVMISDNSAENIALVTSMYDGLLQAGARLCPTVQLEDIRNLQDLTPLKLAAKEGKIEIFRHILQREFSGLSHLSRKFTEWCYGPVRVSLYDLASVDSCEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transient receptor potential cation channel, subfamily V, member 2
别名 230141C3, Osm 9 like TRP channel 2, Osm-9-like TRP channel 2, OTRPC2, Vanilloid receptor-like protein 1
计算分子量 764 aa, 86 kDa
观测分子量~80 kDa
GenBank蛋白编号BC018926
基因名称 TRPV2
Gene ID (NCBI) 51393
RRIDAB_3670763
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y5S1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transient receptor potential vanilloid 2 (TRPV2), also name as VRL-1, plays a critical role in neuronal development, cardiac function, immunity, and cancer (PMID: 31566564). It has been found to play an essential role in sustained intracellular Ca2+ concentration increase (PMID: 31857697). It's reported that TRPV2 is activated by high temperatures, with a threshold of around 52℃ (PMID: 10201375).

实验方案

Product Specific Protocols
WB protocol for TRPV2 antibody 83022-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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