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验证数据展示

  • WB analysis using 68240-1-Ig

    WB analysis using 68240-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68240-1-Ig (PSMA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 68240-1-Ig

    IHC staining of human cervical cancer using 68240-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68240-1-Ig (PSMA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA2 antibody included
  • IHC staining of human cervical cancer using 68240-1-Ig

    IHC staining of human cervical cancer using 68240-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68240-1-Ig (PSMA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA2 antibody included

PSMA2 Monoclonal antibody

PSMA2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68240-1-Ig

产品说明书

CloneNo. 2H4A10

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, pig, rat

应用

WB, IHC, ELISA

别名

HC3, Macropain subunit C3, MU, PMSA2, Proteasome component C3, PSC2, PSC3, PSMA2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, A431 cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PSMA2 Ready-To-Use IHC Kit. PSMA2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68240-1-Ig targets PSMA2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, pig, rat
免疫原 PSMA2 fusion protein Ag5837 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 2
别名 HC3, Macropain subunit C3, MU, PMSA2, Proteasome component C3, PSC2, PSC3, PSMA2
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC047697
基因名称 PSMA2
Gene ID (NCBI) 5683
RRIDAB_2935327
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP25787
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA2 (Proteasome subunit alpha type-2) is the essential subunit that contributes to the complete assembly of 20S proteasome complex, involved in the proteolytic degradation of most intracellular proteins. It is required in spermatogenesis and generation of a subset of MHC class I-presented antigenic peptides(PMID: 34711951, PMID: 27342858). Dysregulation of PSMA2 degradation leads to tumorigenesis including cervical cancer (PMID:33684886).

实验方案

Product Specific Protocols
WB protocol for PSMA2 antibody 68240-1-IgDownload protocol
IHC protocol for PSMA2 antibody 68240-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols