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验证数据展示

  • WB analysis of human plasma using 68020-1-Ig

    WB analysis of human plasma using 68020-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 68020-1-Ig

    IHC staining of human kidney using 68020-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AGT antibody included
  • IHC staining of human kidney using 68020-1-Ig

    IHC staining of human kidney using 68020-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AGT antibody included
  • IF Staining of HepG2 using 68020-1-Ig

    IF Staining of HepG2 using 68020-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AGT antibody (68020-1-Ig, Clone: 1B2F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of human placenta using 68020-1-Ig

    WB analysis of human placenta using 68020-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Angiotensinogen/AGT Monoclonal antibody

Angiotensinogen/AGT Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68020-1-Ig

产品说明书

CloneNo. 1B2F5

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

WB, IHC, IF/ICC, ELISA

别名

AGT, Angiotensinogen, Serpin A8, SERPINA8, 1B2F5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma, HuH-7 cells, THP-1 cells, human placenta tissue, pig plasma
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AGT Ready-To-Use IHC Kit. AGT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68020-1-Ig targets Angiotensinogen/AGT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, pig
免疫原 Angiotensinogen/AGT fusion protein Ag16658 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
别名 AGT, Angiotensinogen, Serpin A8, SERPINA8, 1B2F5
计算分子量 485 aa, 53 kDa
观测分子量52 kDa
GenBank蛋白编号BC011519
基因名称 AGT
Gene ID (NCBI) 183
RRIDAB_2918765
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01019
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

实验方案

Product Specific Protocols
WB protocol for Angiotensinogen/AGT antibody 68020-1-IgDownload protocol
IHC protocol for Angiotensinogen/AGT antibody 68020-1-IgDownload protocol
IF protocol for Angiotensinogen/AGT antibody 68020-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols