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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human plasma using 68020-1-Ig (same clone as 68020-1-PBS)

    WB analysis of human plasma using 68020-1-Ig (same clone as 68020-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

    IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGT antibody included
  • IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

    IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGT antibody included
  • IF Staining of HepG2 using 68020-1-Ig (same clone as 68020-1-PBS)

    IF Staining of HepG2 using 68020-1-Ig (same clone as 68020-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AGT antibody (68020-1-Ig, Clone: 1B2F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 68020-1-Ig (same clone as 68020-1-PBS)

    WB analysis of human placenta using 68020-1-Ig (same clone as 68020-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

Angiotensinogen/AGT Monoclonal antibody, PBS Only

Angiotensinogen/AGT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68020-1-PBS

产品说明书

CloneNo. 1B2F5

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AGT, Angiotensinogen, Serpin A8, SERPINA8, 1B2F5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AGT Ready-To-Use IHC Kit. AGT primary antibody included.

产品信息

68020-1-PBS targets Angiotensinogen/AGT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原 Angiotensinogen/AGT fusion protein Ag16658 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
别名 AGT, Angiotensinogen, Serpin A8, SERPINA8, 1B2F5
计算分子量 485 aa, 53 kDa
观测分子量52 kDa
GenBank蛋白编号BC011519
基因名称 AGT
Gene ID (NCBI) 183
RRIDAB_2918765
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01019
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.