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Species Reactivity
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验证数据展示

  • WB analysis using 67886-1-Ig

    WB analysis using 67886-1-Ig

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 67886-1-Ig (KDM1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of SW 1990 using 67886-1-Ig

    FC experiment of SW 1990 using 67886-1-Ig

    1X10^6 SW 1990 cells were intracellularly stained with 0.4 ug Anti-HUMAN KDM1B (67886-1-Ig, Clone:1E1A3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

KDM1B Monoclonal antibody

KDM1B Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67886-1-Ig

产品说明书

CloneNo. 1E1A3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

AOF1, LSD2, C6orf193, 1E1A3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, K-562 cells, C6 cells, NIH/3T3 cells, A549 cells, HeLa cells, Jurkat cells
Positive FC (Intra) detected inSW 1990 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67886-1-Ig targets KDM1B in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KDM1B fusion protein Ag30672 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 amine oxidase (flavin containing) domain 1
别名 AOF1, LSD2, C6orf193, 1E1A3
计算分子量 92 kDa
观测分子量 92 kDa
GenBank蛋白编号NM_001364614
基因名称 KDM1B
Gene ID (NCBI) 221656
RRIDAB_2918643
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NB78
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KDM1B antibody 67886-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols